3D Histology Evaluation of Dermatologic Surgery by Helmut Breuninger, Patrick Adam

By Helmut Breuninger, Patrick Adam

There hasn’t been a e-book referring to "Microscopically managed surgical procedure" released and it is important to post a ebook that info the entire varied phrases and technique utilized in microscopically managed surgical procedure. The objective is to create a pragmatic, concise and straightforward clarification of 3D-histology with workflows and unique illustrative fabric for dermatologists. it's for that reason designed to be a goal-oriented guide instead of an exhaustive reference paintings. it's going to give you the crucial details for all operating with sufferers present process this team of treatments.

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3c). Handling of Tumor Specimens of ca. 3 Dividing a tumor in two margin strips and base, embedding in paraffin cassettes following a rule and resulting flipped over histological slides To place the pieces into the embedding cassettes, some rules should be followed to facilitate topographic orientation. First the pieces of the margin are turned 90° (Fig. 3d) and placed into the cassette with the outside of margin facing down. , clockwise from the right to the left with the epidermis facing to the right.

Thickness and size should be suitable for processing. 1). The thickness of the tissue which has to be embedded should be 2–4 mm. Also, in H. Breuninger, P. 1007/978-1-4471-4438-0_4, © Springer-Verlag London 2013 45 46 Chapter 4. Dividing Tumors and Topographical Orientation all instances the divisions have to allow for a full reconstruction of the 3D margin. Otherwise, a precise localization of the tumor outgrowth on the patient will be impossible to achieve. Therefore, it is vital to mark division planes not to lose orientation for required re-excisions.

Otherwise, a precise localization of the tumor outgrowth on the patient will be impossible to achieve. Therefore, it is vital to mark division planes not to lose orientation for required re-excisions. As mentioned before, also the choice of oblique or vertical excision technique influences the way how the tumor is divided. The separation of the tumor margins and dividing in parts fitting for further processing has to be done by the surgeon or pathologist as shown in Chap. 2. In the following these different methods, including important methods for topographic orientation, will be illustrated and discussed with examples for mid-sized and large tumor excisions.

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