1996 International Symposium on Bacterial by Gerrit Eggink, National Research Council Canada

By Gerrit Eggink, National Research Council Canada

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Complexes of the triolide from (R)-3-hydroxybutanoic acid with sodium, potassium, and barium salts. Helv. Chim. Acta76: 25812601. and Reusch, R. N. 1994. Isolation and 1H-NMR spectroscopic identification of poly(3-hydroxybutanoate) from prokaryotic and eukaryotic organisms. Eur. J. Biochem. 224: 317328. , Saitoh, M. Namba, T. 1991. Volcanic products of polyphosphate and its relevance to prebiotic evolution. Nature 352: 516517. Page 20 Blends of Natural and Synthetic PHAs Mariastella Scandola1 Dipartimento di Chimica 'G.

This prevalent form of PHB has been termed cPHB to distinguish it from the high molecular weight granular inclusions (gPHB). The bacterial granules of gPHB and its gPHA homologs have attained considerable commercial importance as ingredients of biodegradable plastics; it now appears that an understanding of the nature and functions of cPHB as an integral cell constituent is of no less significance. * Author to whom all correspondence should be addressed Tel. No. edu Page 11 cPHB Complexes in Bacteria cPHB was first discovered in association with inorganic polyphosphate in the plasma membranes of Azotobacter vinelandii, and subsequently in the plasma membranes of Bacillus subtilus, Haemophilus influenzae and Escherichia coli ( (Reusch and Sadoff 1983; Reusch et al.

As the more complex organic polyphosphatesRNA and DNAevolved, PHB may have formed a partnership with proteins to protect these polymeric anions, transport them within the cell, and transfer them between cells. This view places PHB at the very origins of life. Acknowledgments This study was supported by NIH Grant GM333375, NSF Grant MCB 9507282, and a REF-Protein Structure, Function and Design Award from the MSU Biotechnology Research Center. Anderson, A. J. and Dawes, E. A. 1990. Occurrence, metabolism, metabolic role, and industrial uses of bacterial polyhydroxyalkanoates.

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